Caspase 9 antibody (pTyr153)

Catalog No. ABIN1531532

The Rabbit Polyclonal anti-Caspase 9 antibody has been validated for WB, ELISA and IF. It is suitable to detect Caspase 9 in samples from Human.

Quick Overview for Caspase 9 antibody (pTyr153) (ABIN1531532)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Caspase 9 Antibody

Binding Specificity: AA 119-168, pTyr153

Specificity:

Caspase 9 (Phospho-Tyr153) Antibody detects endogenous levels of Caspase 9 only when phosphorylated at Tyr153.

PhosphorylationH:Y153

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 9 around the phosphorylation site of Tyr153.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000

Comment:

Unigene-Number: Hs.329502 (NCBI Gene Symbol: CASP9)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase 9

Background:

Synonyms: APAF-3, Apoptotic protease Mch-6, Apoptotic protease activating factor 3, CASP-9, Caspase-9, Caspase-9 precursor, Caspase9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6, RNCASP9

NCBI Gene Symbol: CASP9

Molecular Weight: 46 kDa

Gene ID: 842

OMIM: 602234

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity