CD226 antibody (pSer329)

Catalog No. ABIN482153

The Rabbit Polyclonal anti-CD226 antibody is suitable to detect CD226 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CD226 antibody (pSer329) (ABIN482153)

Target: CD226

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD226 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD226 Antibody

Binding Specificity: AA 287-336, pSer329

Specificity:

CD226/DNAM-1 (Phospho-Ser329) Antibody detects endogenous levels of CD226/DNAM-1 only when phosphorylated at Ser329.

PhosphorylationH:S329 M:S326

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CD226/DNAM-1 around the phosphorylation site of Ser329.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Comment:

Unigene-Number: Hs.660130 (NCBI Gene Symbol: CD226)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CD226

Target: CD226

Alternative Name: CD226/DNAM-1

Background:

Synonyms: CD226, DNAM1, DNAX accessory molecule-1, PTA1, TLiSA1, adhesion glycoprotein, platelet and T cell activation antigen 1

NCBI Gene Symbol: CD226

Molecular Weight: 38 kDa

Gene ID: 10666

OMIM: 605397

UniProt: Q15762

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints