Cyclin E2 antibody (pThr392)

Catalog No. ABIN1531565

The Rabbit Polyclonal anti-Cyclin E2 antibody is suitable to detect Cyclin E2 in samples from Human and Mouse. It has been validated for IF and ELISA.

Quick Overview for Cyclin E2 antibody (pThr392) (ABIN1531565)

Target: Cyclin E2 (CCNE2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E2 antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA

Product Details anti-Cyclin E2 Antibody

Binding Specificity: AA 355-404, pThr392

Purpose: Cyclin E2 (phospho Thr392) Antibody

Specificity: Phospho-Cyclin E2 (T392) Polyclonal Antibody detects endogenous levels of Cyclin E2 protein only when phosphorylated at T392.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin E2 around the phosphorylation site of Thr392. AA range:355-404

Isotype: IgG

Application Details

Application Notes: IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Cyclin E2

Target: Cyclin E2 (CCNE2)

Alternative Name: Cyclin E2

Background:

Synonyms: CCNE2, G1/S-specific cyclin-E2

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008],

Gene Name: CCNE2

Protein Name: G1/S-specific cyclin-E2

Molecular Weight: 46 kDa

Gene ID: 9134

OMIM: 603775

UniProt: O96020

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases