CDK9 antibody (pThr186)

Catalog No. ABIN1531647

The Rabbit Polyclonal anti-CDK9 antibody (ABIN1531647) specifically detects CDK9 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CDK9 antibody (pThr186) (ABIN1531647)

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDK9 Antibody

Binding Specificity: AA 152-201, pThr186

Purpose: Cdk9 (phospho Thr186) Antibody

Specificity: Phospho-Cdk9 (T186) Polyclonal Antibody detects endogenous levels of Cdk9 protein only when phosphorylated at T186.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CDK9 around the phosphorylation site of Thr186. AA range:152-201

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Alternative Name: CDK9

Background:

Synonyms: CDK9, CDC2L4, TAK, Cyclin-dependent kinase 9, C-2K, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, Tat-associated kinase complex catalytic subunit

Background: cyclin dependent kinase 9(CDK9) Homo sapiens The protein encoded byThis gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008],

Gene Name: CDK9

Protein Name: Cyclin-dependent kinase 9

Molecular Weight: 42 kDa

Gene ID: 1025

OMIM: 603251

UniProt: P50750

Pathways: Cell Division Cycle