PIKFYVE antibody (pSer307)

Catalog No. ABIN1531726

This Rabbit Polyclonal antibody specifically detects PIKFYVE in ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PIKFYVE antibody (pSer307) (ABIN1531726)

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIKFYVE antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PIKFYVE Antibody

Binding Specificity: AA 273-322, pSer307

Purpose: PIP5KIII (phospho Ser307) Antibody

Specificity: Phospho-PIP5KIII (S307) Polyclonal Antibody detects endogenous levels of PIP5KIII protein only when phosphorylated at S307.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PIP5K around the phosphorylation site of Ser307. AA range:273-322

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PIKFYVE

Target: PIKFYVE (phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE))

Alternative Name: PIP5K

Background:

Synonyms: PIKFYVE, KIAA0981, PIP5K3, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type

Background: Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring.This gene encodes an enzyme (PIKfyve, also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKf

Gene Name: PIKFYVE

Protein Name: 1-phosphatidylinositol 3-phosphate 5-kinase

Molecular Weight: 237 kDa

Gene ID: 200576

OMIM: 121850

UniProt: Q9Y2I7

Pathways: Inositol Metabolic Process