MUC1 antibody (pTyr1229)

Catalog No. ABIN1531735

This Rabbit Polyclonal antibody specifically detects MUC1 in IHC, WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MUC1 antibody (pTyr1229) (ABIN1531735)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-MUC1 Antibody

Binding Specificity: AA 1201-1250, pTyr1229

Purpose: Mucin 1 (phospho Tyr1229) Antibody

Specificity: Phospho-Mucin 1 (Y1229) Polyclonal Antibody detects endogenous levels of Mucin 1 protein only when phosphorylated at Y1229.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human CD227/MUC1 around the phosphorylation site of Tyr1229. AA range:1201-1250

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: CD227/MUC1

Background:

Synonyms: MUC1, PUM, Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated ep

Background: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate sp

Gene Name: MUC1

Protein Name: Mucin-1 (MUC-1) (Breast carcinoma-associated antigen DF3) (Carcinoma-associated mucin) (Episialin) (H23AG) (Krebs von den Lungen-6) (KL-6) (PEMT) (Peanut-reactive urinary mucin) (PUM) (Polymorphic epithelial mucin) (PEM) (Tumor-associated epithelial membrane antigen) (EMA) (Tumor-associated mucin) (CD antigen CD227) [Cleaved into: Mucin-1 subunit alpha (MUC1-NT) (MUC1-alpha)Mucin-1 subunit beta (MUC1-beta) (MUC1-CT)], CA 15-3, CA15 3, CA 15.3 , CA15-3, CA15.3

Molecular Weight: 122 kDa

Gene ID: 4582

OMIM: 113720

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling