Recombinant MUC1 antibody

Catalog No. ABIN6940113

The Rabbit Monoclonal anti-MUC1 antibody (Clone MUC1-2818R) (ABIN6940113) specifically detects MUC1 in IHC, WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant MUC1 antibody (ABIN6940113)

Target: MUC1 (Mucin 1 (MUC1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: MUC1-2818R

Product Details anti-MUC1 Antibody

Purpose: Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody

Specificity: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human milk-fat globule membranes (HMFGM)

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Application: ELISA (For coating use Ab at 2-5 μg/mL, order Ab without BSA) (Very good capturing Ab), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1

Background:

Breast carcinoma-associated antigen DF3, CA15-3, H23AG, KL-6, MAM6, Mucin 1 cell surface associated, , Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen

Human Lung, Kidney, Colon, endometrial carcinoma., MCF-7 or MDA-231 cells. Breast, Ovarian

Molecular Weight: 265-400kDa

Gene ID: 4582, 89603

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling