Nibrin antibody (pSer278)

Catalog No. ABIN1531747

This Rabbit Polyclonal antibody specifically detects Nibrin in WB, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Nibrin antibody (pSer278) (ABIN1531747)

Target: Nibrin (NBN)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Nibrin Antibody

Binding Specificity: AA 251-300, pSer278

Purpose: Nibrin (phospho Ser278) Antibody

Specificity: Phospho-Nibrin (S278) Polyclonal Antibody detects endogenous levels of Nibrin protein only when phosphorylated at S278.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Nibrin around the phosphorylation site of Ser278. AA range:251-300

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: Nibrin

Background:

Synonyms: NBN, NBS, NBS1, P95, Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1

Background: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008],

Gene Name: NBN

Protein Name: Nibrin

Molecular Weight: 84 kDa

Gene ID: 4683

OMIM: 114480

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response