RECQL2 antibody (pSer1141)

Catalog No. ABIN1531762

This Rabbit Polyclonal antibody specifically detects RECQL2 in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for RECQL2 antibody (pSer1141) (ABIN1531762)

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RECQL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RECQL2 Antibody

Binding Specificity: AA 1107-1156, pSer1141

Purpose: WRN (phospho Ser1141) Antibody

Specificity: Phospho-WRN (S1141) Polyclonal Antibody detects endogenous levels of WRN protein only when phosphorylated at S1141.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Werner Syndrome Helicase around the phosphorylation site of Ser1141. AA range:1107-1156

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for RECQL2

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Alternative Name: Werner Syndrome Helicase

Background:

Synonyms: WRN; RECQ3; RECQL2; Werner syndrome ATP-dependent helicase; DNA helicase; RecQ-like type 3; RecQ3; Exonuclease WRN; RecQ protein-like 2

Background: Werner syndrome RecQ like helicase(WRN) Homo sapiens This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008],

Gene Name: WRN

Protein Name: Werner syndrome ATP-dependent helicase

Molecular Weight: 162 kDa

Gene ID: 7486

OMIM: 114500

UniProt: Q14191

Pathways: DNA Damage Repair