CHEK1 antibody (pSer345)

Catalog No. ABIN1531801

This Rabbit Polyclonal antibody specifically detects CHEK1 in ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CHEK1 antibody (pSer345) (ABIN1531801)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 310-359, pSer345

Specificity:

Chk1 (Phospho-Ser345) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser345.

PhosphorylationH:S345 M:S345 R:S345

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser345.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHEK1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: Chk1

Background:

Synonyms: CHEK1, Chk1, kinase Chk1

NCBI Gene Symbol: CHEK1

Molecular Weight: 54 kDa

Gene ID: 1111

OMIM: 603078

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair