Nibrin antibody (pSer343)

Catalog No. ABIN1531914

The Rabbit Polyclonal anti-Nibrin antibody (ABIN1531914) specifically detects Nibrin in WB and ELISA. The antibody is reactive with Human and Rat samples.

Quick Overview for Nibrin antibody (pSer343) (ABIN1531914)

Target: Nibrin (NBN)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nibrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Nibrin Antibody

Binding Specificity: AA 310-359, pSer343

Purpose: Nibrin (phospho Ser343) Antibody

Specificity: Phospho-Nibrin (S343) Polyclonal Antibody detects endogenous levels of Nibrin protein only when phosphorylated at S343.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human p95/NBS1 around the phosphorylation site of Ser343. AA range:310-359

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Nibrin

Target: Nibrin (NBN)

Alternative Name: p95/NBS1

Background:

Synonyms: NBN, NBS, NBS1, P95, Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1

Background: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008],

Gene Name: NBN

Protein Name: Nibrin

Molecular Weight: 84 kDa

Gene ID: 4683

OMIM: 114480

UniProt: O60934

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response