NF-kB p65 antibody (pSer276)

Catalog No. ABIN1531923

Cited in 1 publication. The Rabbit Polyclonal anti-NF-kB p65 antibody (ABIN1531923) specifically detects NF-kB p65 in WB, IHC, ELISA, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NF-kB p65 antibody (pSer276) (ABIN1531923)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-NF-kB p65 Antibody

Binding Specificity: AA 249-298, pSer276

Purpose: NFκB-p65 (phospho Ser276) Antibody

Specificity: Phospho-NFκB-p65 (S276) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when phosphorylated at S276.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser276. AA range:249-298

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 μg:mg lysate. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-NF-kB p65 antibody (ABIN1531923)

Shen, Zhao, Zeng, Yan, Chen, Leng, Mu, Zhang: "Inhibition of Pim-1 kinase ameliorates dextran sodium sulfate-induced colitis in mice." in: Digestive diseases and sciences, Vol. 57, Issue 7, pp. 1822-31, (2012) (PubMed).

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: NF-KappaB p65

Background:

Synonyms: RELA, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Gene Name: RELA

Protein Name: Transcription factor p65

Molecular Weight: 60 kDa

Gene ID: 5970

OMIM: 164014

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins