ITCH antibody (pTyr420)

Catalog No. ABIN1532110

The Rabbit Polyclonal anti-ITCH antibody is suitable to detect ITCH in samples from Human and Mouse. It has been validated for ELISA, IHC and IF.

Quick Overview for ITCH antibody (pTyr420) (ABIN1532110)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ITCH Antibody

Binding Specificity: AA 386-435, pTyr420

Purpose: AIP4 (phospho Tyr420) Antibody

Specificity: Phospho-AIP4 (Y420) Polyclonal Antibody detects endogenous levels of AIP4 protein only when phosphorylated at Y420.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. AA range:386-435

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH

Background:

Synonyms: ITCH, E3 ubiquitin-protein ligase Itchy homolog, Itch, Atrophin-1-interacting protein 4, AIP4, NFE2-associated polypeptide 1, NAPP1

Background: itchy E3 ubiquitin protein ligase(ITCH) Homo sapiens This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],

Gene Name: ITCH

Protein Name: E3 ubiquitin-protein ligase Itchy homolog

Molecular Weight: 102 kDa

Gene ID: 83737

OMIM: 606409

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events