Ephrin B1/B2/B3 (AA 290-339), (pTyr324) antibody

Catalog No. ABIN1532118

Product Details

Target: Ephrin B1/B2/B3

Binding Specificity: AA 290-339, pTyr324

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity:

Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324.

PhosphorylationH:Y324/311/318 M:Y323/314/318 R:Y323/-/318

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.144700, Hs.149239, Hs.26988 (NCBI Gene Symbol: EFNB1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details

Target: Ephrin B1/B2/B3

Background:

Synonyms: CEK5 ligand, CEK5 receptor ligand, CEK5-L, CEL5-L, EFL-3, EFNB1, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPL2, EPLG2, Ephrin-B1 precursor, LERK-2, LERK2, STRA1, STRA1 protein, kinase ephrin-B1

NCBI Gene Symbol: EFNB1

Molecular Weight: 38 kDa

Gene ID: 1947, 1948, 1949

OMIM: 300035

UniProt: P98172, P52799, Q15768