Ephrin B1/B2/B3 (pTyr324) antibody

Catalog No. ABIN7220130

The Rabbit Polyclonal anti-Ephrin B1/B2/B3 antibody (ABIN7220130) specifically detects Ephrin B1/B2/B3 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Ephrin B1/B2/B3 (pTyr324) antibody (ABIN7220130)

Target: Ephrin B1/B2/B3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details

Binding Specificity: pTyr324

Purpose: Rabbit Anti-Ephrin-B1/2/3 (phospho Tyr324) Polyclonal Antibody

Specificity: Phospho-Ephrin-B1/2/3 (Y324) Polyclonal Antibody detects endogenous levels of Ephrin-B1/2/3 protein only when phosphorylated at Y324.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Ephrin-B1/2/3 Phospho-Tyr324

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Ephrin B1/B2/B3

Alternative Name: Ephrin-B1/2/3

Background: EFNB1, EFL3, EPLG2, LERK2, Ephrin-B1, EFL-3, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2, LERK-2, EFNB2, EPLG5, HTKL, LERK5, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-L, EFNB3, EPThe ephrin B1 encoded by EFNB3 is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.

Molecular Weight: 46kD

Gene ID: 1947, 1948, 1949

UniProt: P98172, P52799, Q15768