Ephrin B1/B2/B3 (pTyr324) antibody

Catalog No. ABIN6255887

This Rabbit Polyclonal antibody specifically detects Ephrin B1/B2/B3 in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Ephrin B1/B2/B3 (pTyr324) antibody (ABIN6255887)

Target: Ephrin B1/B2/B3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details

Binding Specificity: pTyr324

Specificity: Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at Tyr324

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Phospho-Ephrin B1/B2/B3 (Tyr324)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000 IHC 1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details

Target: Ephrin B1/B2/B3

Background:

Description: Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

Gene: EFNB1

Molecular Weight: 38 kDa

Gene ID: 1947

UniProt: P98172, P52799, Q15768