BRCA1 antibody (pSer1457)

Catalog No. ABIN1532128

The Rabbit Polyclonal anti-BRCA1 antibody is suitable to detect BRCA1 in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for BRCA1 antibody (pSer1457) (ABIN1532128)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 1423-1472, pSer1457

Purpose: BRCA1 (phospho Ser1457) Antibody

Specificity: Phospho-BRCA1 (S1457) Polyclonal Antibody detects endogenous levels of BRCA1 protein only when phosphorylated at S1457.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human BRCA1 around the phosphorylation site of Ser1457. AA range:1423-1472

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background:

Synonyms: BRCA1, RNF53, Breast cancer type 1 susceptibility protein, RING finger protein 53

Background: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript varian

Gene Name: BRCA1

Protein Name: Breast cancer type 1 susceptibility protein

Molecular Weight: 207 kDa

Gene ID: 672

OMIM: 113705

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus