Girdin antibody (pSer1417)

Catalog No. ABIN1532146

The Rabbit Polyclonal anti-Girdin antibody is suitable to detect Girdin in samples from Human and Mouse. It has been validated for ELISA, IHC and IF.

Quick Overview for Girdin antibody (pSer1417) (ABIN1532146)

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Girdin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Girdin Antibody

Binding Specificity: AA 1383-1432, pSer1417

Purpose: Girdin (phospho Ser1417) Antibody

Specificity: Phospho-Girdin (S1417) Polyclonal Antibody detects endogenous levels of Girdin protein only when phosphorylated at S1417.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Girdin around the phosphorylation site of Ser1417. AA range:1383-1432

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Girdin

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Alternative Name: Girdin

Background:

Synonyms: CCDC88A, APE, GRDN, KIAA1212, Girdin, Akt phosphorylation enhancer, APE, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, Girders of actin filament, Hook-related protein 1, HkRP1

Background: This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011],

Gene Name: CCDC88A

Protein Name: Girdin

Molecular Weight: 216 kDa

Gene ID: 55704

OMIM: 609736

UniProt: Q3V6T2