ZYX antibody (pSer142)

Catalog No. ABIN1532180

This Rabbit Polyclonal antibody specifically detects ZYX in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ZYX antibody (pSer142) (ABIN1532180)

Target: ZYX (Zyxin (ZYX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZYX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ZYX Antibody

Binding Specificity: AA 108-157, pSer142

Purpose: Zyxin (phospho Ser142) Antibody

Specificity: Phospho-Zyxin (S142) Polyclonal Antibody detects endogenous levels of Zyxin protein only when phosphorylated at S142.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Zyxin around the phosphorylation site of Ser142. AA range:108-157

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ZYX

Target: ZYX (Zyxin (ZYX))

Alternative Name: Zyxin

Background:

Synonyms: ZYX, Zyxin, Zyxin-2

Background: Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008],

Gene Name: ZYX

Protein Name: Zyxin

Molecular Weight: 61 kDa

Gene ID: 7791

OMIM: 602002

UniProt: Q15942