Lamin A/C antibody (pSer22)

Catalog No. ABIN1532183

This Rabbit Polyclonal antibody specifically detects Lamin A/C in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for Lamin A/C antibody (pSer22) (ABIN1532183)

Target: Lamin A/C (LMNA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Lamin A/C Antibody

Binding Specificity: AA 2-51, pSer22

Specificity:

Lamin A (Phospho-Ser22) Antibody detects endogenous levels of Lamin A only when phosphorylated at Ser22.

PhosphorylationH:S22 M:S22 R:S22

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Lamin A around the phosphorylation site of Ser22.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 ELISA: 1:1000

Comment:

Unigene-Number: Hs.594444 (NCBI Gene Symbol: LMNA)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-Lamin A/C antibody (ABIN1532183)

Häge, Sonntag, Svrlanska, Borst, Stilp, Horsch, Müller, Kropff, Milbradt, Stamminger, Schlötzer-Schrehardt, Marschall: "Phenotypical Characterization of the Nuclear Egress of Recombinant Cytomegaloviruses Reveals Defective Replication upon ORF-UL50 Deletion but Not pUL50 Phosphosite Mutation." in: Viruses, Vol. 13, Issue 2, (2021) (PubMed).

Schütz, Steingruber, Socher, Müller, Wagner, Kögel, Sticht, Marschall: "Functional Relevance of the Interaction between Human Cyclins and the Cytomegalovirus-Encoded CDK-Like Protein Kinase pUL97." in: Viruses, Vol. 13, Issue 7, (2021) (PubMed).

Häge, Büscher, Pakulska, Hahn, Adrait, Krauter, Borst, Schlötzer-Schrehardt, Couté, Plachter, Marschall: "The Complex Regulatory Role of Cytomegalovirus Nuclear Egress Protein pUL50 in the Production of Infectious Virus." in: Cells, Vol. 10, Issue 11, (2021) (PubMed).

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A

Background:

Synonyms: 70 kDa lamin, LAMA, LMN1, LMNA

NCBI Gene Symbol: LMNA

Molecular Weight: 74 kDa

Gene ID: 4000

OMIM: 115200

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus