H2AFX antibody (AA 94-143)

Catalog No. ABIN1532893

The Rabbit Polyclonal anti-H2AFX antibody has been validated for WB, IHC and ELISA. It is suitable to detect H2AFX in samples from Human, Mouse and Rat.

Quick Overview for H2AFX antibody (AA 94-143) (ABIN1532893)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-H2AFX Antibody

Binding Specificity: AA 94-143

Specificity:

Histone H2A.X Antibody detects endogenous levels of total Histone H2A.X protein.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H2A.X.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.477879 (NCBI Gene Symbol: H2AFX)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: Histone H2A.X

Background:

Synonyms: H2A.X, H2AFX, H2a/x, HIST5-2AX, Histone H2A.X

NCBI Gene Symbol: H2AFX

Molecular Weight: 15 kDa

Gene ID: 3014

OMIM: 601772

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus