Cyclin E1 antibody (AA 91-140)

Catalog No. ABIN1533249

The Rabbit Polyclonal anti-Cyclin E1 antibody is suitable to detect Cyclin E1 in samples from Human, Rat, Mouse and Pig. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Cyclin E1 antibody (AA 91-140) (ABIN1533249)

Target: Cyclin E1 (CCNE1)

Reactivity: Human, Rat, Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Cyclin E1 Antibody

Binding Specificity: AA 91-140

Purpose: Cyclin E1 Antibody

Specificity: Cyclin E1 Polyclonal Antibody detects endogenous levels of Cyclin E1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin E1. AA range:91-140

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: Cyclin E1

Background:

Synonyms: CCNE1, CCNE, G1/S-specific cyclin-E1

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in

Gene Name: CCNE1

Protein Name: G1/S-specific cyclin-E1

Molecular Weight: 47 kDa

Gene ID: 898

OMIM: 123837

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases