ERCC1 antibody (AA 141-190)

Catalog No. ABIN1533265

The Rabbit Polyclonal anti-ERCC1 antibody (ABIN1533265) specifically detects ERCC1 in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for ERCC1 antibody (AA 141-190) (ABIN1533265)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ERCC1 Antibody

Binding Specificity: AA 141-190

Purpose: ERCC1 Antibody

Specificity: ERCC1 Polyclonal Antibody detects endogenous levels of ERCC1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ERCC1. AA range:141-190

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1

Background:

Synonyms: ERCC1; DNA excision repair protein ERCC-1

Background: The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein ge

Gene Name: ERCC1

Protein Name: DNA excision repair protein ERCC-1

Molecular Weight: 32 kDa

Gene ID: 2067

OMIM: 126380

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response