FHIT antibody (AA 81-130)

Catalog No. ABIN1533274

The Rabbit Polyclonal anti-FHIT antibody (ABIN1533274) specifically detects FHIT in ELISA, WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FHIT antibody (AA 81-130) (ABIN1533274)

Target: FHIT (Fragile Histidine Triad (FHIT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHIT antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FHIT Antibody

Binding Specificity: AA 81-130

Purpose: Fhit Antibody

Specificity: Fhit Polyclonal Antibody detects endogenous levels of Fhit protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FHIT. AA range:81-130

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FHIT

Target: FHIT (Fragile Histidine Triad (FHIT))

Alternative Name: FHIT

Background:

Synonyms: FHIT; Bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; AP3Aase; Diadenosine 5'; 5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein

Background: This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009],

Gene Name: FHIT

Protein Name: Bis(5'-adenosyl)-triphosphatase

Molecular Weight: 16 kDa

Gene ID: 2272

OMIM: 601153

UniProt: P49789