Osteopontin antibody (AA 261-310)

Catalog No. ABIN1533364

The Rabbit Polyclonal anti-Osteopontin antibody (ABIN1533364) specifically detects Osteopontin in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Osteopontin antibody (AA 261-310) (ABIN1533364)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Osteopontin Antibody

Binding Specificity: AA 261-310

Purpose: OPN Antibody

Specificity: OPN Polyclonal Antibody detects endogenous levels of OPN protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Osteopontin. AA range:261-310

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000,IHC 1:100-500,IF ICC 1:100-500,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: Osteopontin

Background:

Synonyms: SPP1, BNSP, OPN, PSEC0156, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin

Background: secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

Gene Name: SPP1

Protein Name: Osteopontin

Molecular Weight: 35 kDa

Gene ID: 6696

OMIM: 166490

UniProt: P10451

Pathways: Regulation of Cell Size