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Vimentin antibody (AA 411-460)

This Rabbit Polyclonal antibody specifically detects Vimentin in WB, IF, ELISA, IHC (p) and ICC. It exhibits reactivity toward Human, Rat and Mouse.It has been mentioned in 1 publication
Catalog No. ABIN1533459
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Quick Overview for Vimentin antibody (AA 411-460) (ABIN1533459)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

  • 426
  • 135
  • 126
  • 55
  • 49
  • 44
  • 43
  • 30
  • 29
  • 19
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  • 12
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  • 2
  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Rat, Mouse

Host

  • 230
  • 180
  • 9
  • 7
  • 5
  • 5
  • 4
  • 2
Rabbit

Clonality

  • 247
  • 194
Polyclonal

Conjugate

  • 265
  • 22
  • 15
  • 12
  • 11
  • 10
  • 6
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  • 5
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  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Vimentin antibody is un-conjugated

Application

  • 358
  • 197
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  • 59
  • 50
  • 42
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  • 1
  • 1
  • 1
  • 1
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  • 1
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Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
  • Binding Specificity

    • 40
    • 32
    • 27
    • 16
    • 14
    • 12
    • 12
    • 11
    • 8
    • 7
    • 7
    • 6
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    • 2
    • 2
    • 2
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 411-460

    Purpose

    VIM Antibody

    Specificity

    VIM Polyclonal Antibody detects endogenous levels of VIM protein.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human Vimentin. AA range:411-460

    Isotype

    IgG
  • Application Notes

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C/1 year

    Expiry Date

    12 months
  • Yu, Zeidel, Hill: "Cellular expression profile for interstitial cells of cajal in bladder - a cell often misidentified as myocyte or myofibroblast." in: PLoS ONE, Vol. 7, Issue 11, pp. e48897, (2012) (PubMed).

  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Synonyms: VIM, Vimentin

    Background: tumor protein p73(TP73) Homo sapiens This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined. [provided by RefSeq, Feb 2011],

    Gene Name: VIM

    Protein Name: Vimentin

    Molecular Weight

    53 kDa

    OMIM

    193060

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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