TNF alpha antibody (AA 141-190)

Catalog No. ABIN1533583

The Rabbit Polyclonal anti-TNF alpha antibody has been validated for ELISA, IHC and IF. It is suitable to detect TNF alpha in samples from Human, Mouse and Rat.

Quick Overview for TNF alpha antibody (AA 141-190) (ABIN1533583)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TNF alpha Antibody

Binding Specificity: AA 141-190

Specificity:

TNFA Antibody detects endogenous levels of total TNFA protein.

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TNFA.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000

Comment:

Unigene-Number: Hs.241570 (NCBI Gene Symbol: TNF)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNFA

Background:

Synonyms: Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2

NCBI Gene Symbol: TNF

Molecular Weight: 25 kDa

Gene ID: 7124

OMIM: 191160

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome