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p53 antibody (acLys317)

The Rabbit Polyclonal anti-p53 antibody has been validated for WB, ELISA and IF. It is suitable to detect p53 in samples from Human, Mouse and Rat.
Catalog No. ABIN1535527

Quick Overview for p53 antibody (acLys317) (ABIN1535527)

Target

See all p53 (TP53) Antibodies
p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity

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Human, Mouse, Rat

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This p53 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunofluorescence (IF)
  • Binding Specificity

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    AA 283-332, acLys317

    Specificity

    p53 (Acetyl-Lys317) Antibody detects endogenous levels of total p53 protein only when acetylated at Lys317.

    AcetylationH:K317 M:K314 R:K315

    Purification

    The antibody was purified from rabbit antiserum by affinity-chromatography using acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.

    Purity

    > 95 %

    Immunogen

    The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys317.

    Isotype

    IgG
  • Application Notes

    WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000

    Comment

    Unigene-Number: Hs.654481 (NCBI Gene Symbol: TP53)

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Stable at -20°C for at least 1 year.

    Expiry Date

    12 months
  • Target

    p53 (TP53) (Tumor Protein P53 (TP53))

    Alternative Name

    p53

    Background

    Synonyms: Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53

    NCBI Gene Symbol: TP53

    Molecular Weight

    43 kDa

    Gene ID

    7157

    OMIM

    133239

    UniProt

    P04637

    Pathways

    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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