Factor VII antibody (Cleaved-Arg212)

Catalog No. ABIN1536130

The Rabbit Polyclonal anti-Factor VII antibody is suitable to detect Factor VII in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Factor VII antibody (Cleaved-Arg212) (ABIN1536130)

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor VII antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Factor VII Antibody

Binding Specificity: AA 171-220, Cleaved-Arg212

Purpose: Cleaved-Factor VII LC (R212) Antibody

Specificity: Cleaved-Factor VII LC (R212) Polyclonal Antibody detects endogenous levels of fragment of activated Factor VII LC protein resulting from cleavage adjacent to R212.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FA7. AA range:171-220

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Factor VII

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Alternative Name: FA7

Background:

Synonyms: F7, Coagulation factor VII, Proconvertin, Serum prothrombin conversion accelerator, SPCA, Eptacog alfa

Background: This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015],

Gene Name: F7

Protein Name: Coagulation factor VII

Molecular Weight: 17 kDa

Gene ID: 2155

OMIM: 227500

UniProt: P08709

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling