Actin antibody

Catalog No. ABIN1536597

Cited in 2+ publications. The Mouse Monoclonal anti-Actin antibody (Clone 337CT30-10-1) (ABIN1536597) specifically detects Actin in WB, IHC (p) and FACS. The antibody is reactive with Human samples.

Quick Overview for Actin antibody (ABIN1536597)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 337CT30-10-1

Product Details anti-Actin Antibody

Predicted Reactivity: B, C, M, Pig, Rb, Rat

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This ACTA1 monoclonal antibody is generated from mouse immunized with ACTA1 recombinant protein.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 1:500-1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: ACTA1 Antibody can be refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Actin antibody (ABIN1536597)

Vuković, Jevtić, Zhang, Stohr, Levy: "Nuclear size is sensitive to NTF2 protein levels in a manner dependent on Ran binding." in: Journal of cell science, Vol. 129, Issue 6, pp. 1115-27, (2017) (PubMed).

Edens, Dilsaver, Levy: "PKC-mediated phosphorylation of nuclear lamins at a single serine residue regulates interphase nuclear size in Xenopus and mammalian cells." in: Molecular biology of the cell, Vol. 28, Issue 10, pp. 1389-1399, (2017) (PubMed).

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Background: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

Molecular Weight: 42051

Gene ID: 58

NCBI Accession: NP_001091

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development