tau antibody (pSer409)

Catalog No. ABIN1573965

Product Details anti-tau Antibody

Target: tau (tau Protein)

Binding Specificity: pSer409

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This tau antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-Tau (Phospho-Ser409) Polyclonal Antibody detects human tau only when phosphorylation occurs at serine 409. It has not yet been tested for other species.

Cross-Reactivity (Details): Reactivity to other species is not tested yet.

Purification: Affinity chromatography using phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide.

Immunogen: Synthetic phospho-peptide (KLH-coupled) corresponding - AA surrounding pSer409 of human tau

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, detection method sensitivity, temperature, length of incubations, and many other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are starting recommendations for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-2 µg/mLOther Applications: user optimized.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: Rabbit Anti-Tau (Phospho-Ser⁴⁰⁹) Polyclonal Antibody should be stored lyophilized until use. It remains stable in lyophilized form for at least two years if stored at 2-8°C. The reconstituted antibody can be stored for 2-3 weeks 2-8°C or for up to two years at -20°C or below. Avoid repeated freezing and thawing cycles.

Expiry Date: 24 months

Target Details for tau

Target: tau (tau Protein)

Alternative Name: tau (tau Products)

Synonyms: DDPAC antibody, FTDP-17 antibody, MAPTL antibody, MSTD antibody, MTBT1 antibody, MTBT2 antibody, PPND antibody, TAU antibody, tau antibody, PHF-tau antibody, microtubule associated protein tau antibody, MAPT antibody

Background: Tau is a microtubule-associated phosphoprotein (MAP) localized in neuronal axons. That promotes tubulin polymerization and microtubules stabilization. Tau proteins constitute a family of six isoforms ranging from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeated regions in the carboxyl terminal of the molecule and the absence or presence of one or two inserts in the portion ofamino-terminal. Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3beta transfection phosphorylates serines 199, 202, 235, 396, 404, and 413, and threonines 205 and 231. These sites are among the major atypical phosphorylation sites of tau. Phosphorylation on these sites reduces the ability of a given tau species to promote microtubule self-assembly. Hyperphosphorylated tau is the major protein of the paired helical filaments (PHFs) which make up the pathological neurofibrillary tangles of Alzheimer-disease (AD). PHFs are also found in the lesions of other central nervous system disorders.Rabbit Anti-Tau (Phospho-Ser409) Polyclonal Antibody is developed in rabbit using synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding phospho-serine 409 of human tau.