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- Target
- PKG-2
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Specificity
- Rabbit Anti-PKG-2 Polyclonal Antibody detects endogenous levels of total human and mouse PKG-2 protein.
- Purification
- Immunoaffinity chromatography
- Immunogen
- Synthetic peptide (KLH-coupled) derived from human PKG-2
- Isotype
- IgG
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- Application Notes
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, detection method sensitivity, temperature, length of incubations, and many other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are starting recommendations for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.5-2 µg/mLOther Applications: user optimized. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4, containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- Target
- PKG-2
- Background
- PKGs are cyclic GMP-dependent protein kinases (also known as cGKs or PRKGs) that are classified into two types, PKG-1 and PKG-2. PKG plays an important stimulatory role in platelet activation. All PKG family members are activated by increased levels of cellular cGMP. cGMP binds to the enzyme- regulatory domain inducing a conformational change which leads to enzyme activation.Rabbit Anti-PKG-2 Polyclonal Antibody is developed in rabbit using synthetic peptide (KLH-coupled) derived from human PKG-2.
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