CTCF antibody (AA 150-200)

Catalog No. ABIN1574169

Product Details anti-CTCF Antibody

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Binding Specificity: AA 150-200

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTCF antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: CTCF Antibody detects endogenous levels of human CTCF. Predicted to react with rat CTCF according to sequence homology.Positive Control: Ramos, K562 and A431

Cross-Reactivity (Details): CTCF Antibody detects endogenous levels of human CTCF. Predicted to react with rat CTCF according to sequence homology.Positive Control: Ramos, K562 and A431

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 150-200 of human CTCF .

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/mL.Other Applications: user optimized.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF (CTCF Products)

Synonyms: im:7152666 antibody, CTCF antibody, xctcf antibody, AW108038 antibody, CCCTC-binding factor antibody, CCCTC-binding factor (zinc finger protein) antibody, CTCF antibody, ctcf antibody, Ctcf antibody

Background: CTCF (CCCTC-binding factor) is a highly conserved zinc finger protein which plays an important role in transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation depending on the DNA context of the binding site. It contains a DNA-binding domain composed of 11 zinc-fingers and the DNA target sequences recognized by CTCF which are fairly long and strikingly diverse. CTCF can be phosphorylated by the protein kinase CK2, as well as poly(ADP-ribosyl)ated. The latter modification regulates its activity as a chromatin insulator.CTCF Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 150-00 of human CTCF (Swiss Prot: P49711).