TIE1 antibody

Catalog No. ABIN1589692

This Mouse Monoclonal antibody specifically detects TIE1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for TIE1 antibody (ABIN1589692)

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 8C9

Product Details anti-TIE1 Antibody

Purpose: TIE-1 antibody

Specificity: Recombinant human soluble TIE-1

Cross-Reactivity (Details): The monoclonal antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types. The antibody can be used for ELISA experiments, Western blotting, FACS and cell sorting.

Characteristics: Chromosomal location: 1p34-p33
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular TIE-1 as the immunizing antigen.

Purification: Mouse IgG1 antibody (#8C9) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Immunogen: Recombinant human soluble TIE-1

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 1-15 μg/mL, Western blotting: Use at 1-2 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: TIE-1

Background: TIE1, TIE, JTK14,TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Gene ID: 7075

NCBI Accession: NM_005424, NP_005415

UniProt: P35590

Pathways: RTK Signaling