VEGFR2/CD309 antibody (Biotin)

Catalog No. ABIN1589717

The Mouse Monoclonal anti-VEGFR2/CD309 antibody has been validated for WB, IHC, ELISA and FACS. It is suitable to detect VEGFR2/CD309 in samples from Human.

Quick Overview for VEGFR2/CD309 antibody (Biotin) (ABIN1589717)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGFR2/CD309 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 3(4H3

Product Details anti-VEGFR2/CD309 Antibody (Biotin)

Purpose: VEGFR-2/KDR antibody

Specificity: Recombinant human soluble extracellular KDR (D7)

Cross-Reactivity (Details): The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.

Characteristics: Chromosomal location: 4q11-q12
The monoclonal antibody was produced with the help of BALB/c mice using recombinant human soluble extracellular KDR (110 kDa) as the immunizing antigen. The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.

Purification: Mouse IgG1 antibody (clone 3 (4H3)) from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol.

Immunogen: Recombinant human soluble extracellular KDR (D7)

Isotype: IgG1

Application Details

Application Notes: FACS: Use at 2-10 μg/mL, Western Blot: Use at 2-5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, BSA (50x), 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Centrifuge vial prior to opening.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: VEGFR-2/KDR

Background: Vascular endothelial growth factor receptor-2 , KDR, FLK1, CD309, VEGF receptor 2, VEGFR2, kinase insert domain protein receptor,VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. Human VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Gene ID: 3791

NCBI Accession: NM_002253, NP_002244

UniProt: P35968

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling