CHEK1 antibody (Internal Region)

Catalog No. ABIN1589998

This Goat Polyclonal antibody specifically detects CHEK1 in WB and ELISA. It exhibits reactivity toward Rat.

Quick Overview for CHEK1 antibody (Internal Region) (ABIN1589998)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CHEK1 Antibody

Binding Specificity: Internal Region

Purpose: CHEK1

Sequence: DQPSDSCQEY SD

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Rat Thymus lysates (calculated MW of 54.4 kDa according to Rat NP_536325.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHEK1

Background: CHEK1 , CHK1 checkpoint homolog (S. pombe) , CHK1, CHK1 checkpoint homolog , Checkpoint, S. pombe, homolog of, 1

Gene ID: 1111

NCBI Accession: NP_001265

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair