CA1 antibody (Biotin)

Catalog No. ABIN1607668

Product Details anti-CA1 Antibody (Biotin)

Target: CA1 (Carbonic Anhydrase I (CA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CA1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Carbonic Anhydrase I from other sources may occur but has not been specifically determined.

Purity: Anti-CARBONIC ANHYDRASE I (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes].

Endotoxin Level: Low Endotoxin : No

Immunogen: Carbonic Anhydrase I [Human Erythrocytes]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-CARBONIC ANHYDRASE I (GOAT) Antibody has been assayed against 1.0 µg of Carbonic Anhydrase I in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher.
ELISA Dilution: 1:20.000 - 1:100.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Alternative Name: Carbonic Anhydrase I (CA1 Products)

Synonyms: CA-I antibody, CAB antibody, Car1 antibody, AW555628 antibody, Ca1 antibody, Car-1 antibody, BG:DS00941.1 antibody, CAH antibody, CG7820 antibody, Dmel\\CG7820 antibody, Gh7 antibody, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 antibody, ATBCA1 antibody, ATSABP3 antibody, BETA CARBONIC ANHYDRASE 1 antibody, F4P13.5 antibody, F4P13_5 antibody, SABP3 antibody, SALICYLIC ACID-BINDING PROTEIN 3 antibody, carbonic anhydrase 1 antibody, CA1 antibody, LOC100135826 antibody, GB15888 antibody, carbonic anhydrase 1 antibody, Carbonic anhydrase 1 antibody, carbonic anhydrase I antibody, carbonic anhydrase antibody, CA1 antibody, Car1 antibody, CAH1 antibody, LOC100135826 antibody, LOC408827 antibody, Tsp_05114 antibody, LOC100153915 antibody, LOC100050192 antibody, Ca1 antibody

Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic Anhydrase 1 (CA1) is closely related to Carbonic Anhydrase 2 (CA2) and Carbonic Anhydrase 3 (CA3), and it is a cytosolic protein that is found at the highest level in erythrocytes. Anti-Carbonic anhydrase I antibody is ideal for investigators involved in serum protein components. Synonyms: Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

Gene ID: 759

UniProt: P00915