ADA antibody (Biotin)

Catalog No. ABIN1607721

Product Details anti-ADA Antibody (Biotin)

Target: ADA (Adenosine Deaminase (ADA))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADA antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Adenosine Deaminase from other sources may occur but has not been specifically determined.

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen].

Endotoxin Level: Low Endotoxin : No

Immunogen: Adenosine Deaminase [Calf Spleen]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: This product has been assayed against 1.0 µg of Adenosine Deaminase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:64.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for ADA

Target: ADA (Adenosine Deaminase (ADA))

Alternative Name: Adenosine Deaminase (ADA Products)

Synonyms: ADA-like antibody, xada antibody, ADA antibody, CG11994 antibody, Dmel\\CG11994 antibody, DrosADA antibody, dADA antibody, zgc:92028 antibody, adenosine deaminase antibody, adenosine deaminase S homeolog antibody, Adenosine deaminase antibody, ADA antibody, Ada antibody, ada.S antibody, ada antibody

Background: Adenosine Deaminase catalyzes the deamination of adenosine to inosine, thus destabilizing double-stranded RNA. Anti-Adenosine Deaminase is a member of the adenosine and AMP deaminases family. This antibody is a factor in purine metabolism and adenosine homeostasis, as well as positively regulating T-cell coactivation through the binding of DPP4. The antibody interacts with DPP4 in the extracellular domain, thus regulating lymphocyte-epithelial cell adhesion. It moderates extracellular adenosine signaling. Enzyme deficiency in Adenosine Deaminase has been associated with severe combined immunodeficiency disease, whereas an overabundance may result in congenital hemolytic anemia. Anti-Adenosine Deaminase is ideal for investigators involved in Enzyme, DNA and RNA research. Synonyms: Adenosine deaminase EC=3.5.4.4 Adenosine aminohydrolase

Gene ID: 280712

UniProt: P56658

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process