Amylase, alpha antibody (Biotin)

Catalog No. ABIN1607777

Supplier Product No.: 200-4645-0100

This anti-Amylase, alpha antibody is a Rabbit Polyclonal antibody detecting Amylase, alpha in WB, ELISA and IP. Suitable for Bacillus amyloliquefaciens.

Quick Overview for Amylase, alpha antibody (Biotin) (ABIN1607777)

Target: Amylase, alpha (AMY)

Reactivity: Bacillus amyloliquefaciens

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amylase, alpha antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Amylase, alpha Antibody (Biotin)

Supplier Product No.: 200-4645-0100

Supplier: Rockland

Purpose: Alpha Amylase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.

Characteristics: Synonyms: rabbit anti-Alpha Amylase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase

Purification: Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: a-Amylase [Bacillus amyloliquefaciens]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Alpha Amylase Biotin Conjugated Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Amylase, alpha

Target: Amylase, alpha (AMY)

Alternative Name: Alpha-amylase

Background: Background: Alpha Amylase is a member of the glycosyl hydrolase 13 family, involved primarily in the hydrolysis of biopolymer residues of 1, 4-alpha-D-glucosidic linkages in polysaccharides. It performs its catalytic function in metabolism of carbohydrates. Anti-Alpha Amylase antibody is ideal for investigators involved in Metabolism, Signal Transduction, and Cancer research.

Gene ID: 7849308

NCBI Accession: WP_013352208

UniProt: P00692