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CPA antibody (Biotin)

CPA Reactivity: Cow WB, ELISA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1607785
  • Target See all CPA products
    CPA (Carboxypeptidase A (CPA))
    Reactivity
    • 6
    • 1
    • 1
    Cow
    Host
    • 7
    Rabbit
    Clonality
    • 7
    Polyclonal
    Conjugate
    • 4
    • 2
    • 1
    This CPA antibody is conjugated to Biotin
    Application
    • 6
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA
    Cross-Reactivity (Details)
    Cross reactivity against Carboxypeptidase A from other sources may occur but have not been specifically determined.
    Purity
    Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Carboxypeptidase A [Bovine Pancreas]
    Immunogen Type: Native Protein
    Isotype
    IgG
  • Application Notes
    Anti-Carboxypeptidase A has been assayed against 1.0 µg of Carboxypeptidase A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:55.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:25.000
    Western Blot Dilution: 1:500 - 1:3.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    CPA (Carboxypeptidase A (CPA))
    Alternative Name
    Carboxypeptidase A (CPA Products)
    Background
    Anti-Carboxypeptidase A antibody catalyzes the release of C-terminal amino acids with little or no action with –Asp, -Glu, -Arg, -Lys or –Pro. This antibody is ideal for investigators interested in Metabolism, Cancer, Signal Transduction, or Cell Biology research. Synonyms: Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
    Gene ID
    286762
    UniProt
    P00730
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