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PCK1 antibody (Biotin)

PCK1 Reactivity: Zea mays WB, ELISA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1607799
  • Target See all PCK1 Antibodies
    PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
    Reactivity
    • 80
    • 55
    • 30
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Zea mays
    Host
    • 94
    • 9
    • 2
    • 2
    Rabbit
    Clonality
    • 91
    • 16
    Polyclonal
    Conjugate
    • 49
    • 8
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This PCK1 antibody is conjugated to Biotin
    Application
    • 85
    • 37
    • 28
    • 14
    • 14
    • 13
    • 10
    • 9
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Cross-Reactivity (Details)
    Cross reactivity against Phospho-enol-Pyruvate-Carboxylase from other sources may occur but have not been specifically determined.
    Purity
    Anti-Phospho Enol Pyruvate Carboxylase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate-Carboxylase [Maize].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Phospho-enol-pyruvate Carboxylase [Maize Leaves]
    Immunogen Type: Native Protein
    Isotype
    IgG
    Top Product
    Discover our top product PCK1 Primary Antibody
  • Application Notes
    Anti-Phospho Enol Pyruvate Carboxylase antibody has been assayed against 1.0 µg of Phospho-enol-Pyruvate-Carboxylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:12.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:25.000
    Western Blot Dilution: 1:500 - 1:3.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
    Alternative Name
    Phospho Enol Pyruvate Carboxylase (PCK1 Products)
    Synonyms
    PEPCK-C antibody, PEPCK1 antibody, PEPCKC antibody, PEPCK antibody, PEPCK-M antibody, PEPCK2 antibody, 1810010O14Rik antibody, 9130022B02Rik antibody, cb856 antibody, cb924 antibody, fj93h11 antibody, zgc:63869 antibody, wu:fc51c05 antibody, wu:fj93h11 antibody, pepck-c antibody, pepck1 antibody, pepckc antibody, PCK1 antibody, PPCK1 antibody, AI265463 antibody, Pck-1 antibody, GTP antibody, PCK antibody, Pepck antibody, RATPEPCK antibody, Ppc1C antibody, PHOSPHOENOLPYRUVATE CARBOXYKINASE antibody, T28I19.150 antibody, T28I19_150 antibody, phosphoenolpyruvate carboxykinase 1 antibody, 143299_at antibody, CG10924 antibody, CG17725 antibody, Dmel\\CG17725 antibody, Dromel_CG17725_FBtr0086701_pepck_mORF antibody, dPEPCK antibody, pepck antibody, PEPC antibody, PEPCase antibody, ppc antibody, phosphoenolpyruvate carboxykinase 1 antibody, phosphoenolpyruvate carboxykinase 2, mitochondrial antibody, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody, phosphoenolpyruvate carboxykinase 1 (soluble) antibody, phosphoenolpyruvate carboxykinase 1 S homeolog antibody, phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody, phosphoenolpyruvate carboxykinase 1, cytosolic antibody, phosphoenolpyruvate carboxylase 7 antibody, Phosphoenolpyruvate carboxykinase antibody, phosphoenolpyruvate carboxylase antibody, PCK1 antibody, PCK2 antibody, Pck2 antibody, pck1 antibody, pck1.S antibody, LOC100634531 antibody, Pck1 antibody, pep7 antibody, Pepck antibody, LOC107777405 antibody
    Background
    Anti-Phospho Enol Pyruvate Carboxylase antibody detects PEP. Phosphoenolpyruvate carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate. This reaction is used for carbon fixation in so-called Synonyms: PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody
    Gene ID
    542372
    UniProt
    B8XPZ2
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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