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- Target See all Esterase products
- Esterase
- Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Esterase antibody is conjugated to Biotin
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Application
- ELISA, Western Blotting (WB)
- Cross-Reactivity (Details)
- Cross reactivities against Esterase from other sources may occur but have not been specifically determined.
- Purity
- Anti-Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
- Endotoxin Level
- Low Endotoxin : No
- Immunogen
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Esterase [Porcine Liver]
Immunogen Type: Native Protein - Isotype
- IgG
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anti-Esterase antibody (HRP)
Reactivity: Pig ELISA, WB, IHC Host: Rabbit Polyclonal HRP
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- Application Notes
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Anti-Esterase has been assayed against 1.0 µg of Esterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 10 mg/mL
- Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - Handling Advice
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- Expiry Date
- Expiration date is one (1) year from date of opening.
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- Target
- Esterase
- Abstract
- Esterase Products
- Synonyms
- Carboxylic ester hydrolase antibody, T28C12.4 antibody, K11G9.1 antibody, K07C11.4 antibody, F15A8.6 antibody, K11G9.2 antibody, K11G9.3 antibody, T28C12.5 antibody
- Background
- Anti-Esterase antibody is involved in the detoxification of xenobiotics, as well as in the activation of ester and amide prodrugs. The antibody is inactive on long-chain triacylglycerols and diacylglycerol, but active towards triacylglycerides composed with short-chain fatty acids from C2 to C6 and monoacylglycerols containing fatty acids from C2 to C12. Esterase hydrolyzes aromate and alkyl esters and vitamin A acetate, the rate of hydrolysis varies due to the hydrolyzing substances. Anti-Esterase antibody member of the type-B carboxylesterase/lipase family. Synonyms: Liver carboxylesterase, EC=3.1.1.1, Proline-beta-naphthylamidase, Retinyl ester hydrolase
- Gene ID
- 100626873, 343791015
- UniProt
- Q29550
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