APP antibody (C-Term)

Catalog No. ABIN1607877

Supplier Product No.: 600-401-j10

The Rabbit Polyclonal anti-APP antibody (ABIN1607877) specifically detects APP in WB, ELISA, IHC and FM. The antibody is reactive with Human, Mouse, Rat and Primate samples.

Quick Overview for APP antibody (C-Term) (ABIN1607877)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human, Mouse, Rat, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Product Details anti-APP Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-j10

Supplier: Rockland

Purpose: Beta Amyloid protein 40 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest reactivity with human, sheep, rabbit, canine, bovine, pig, guinea pig, and monkey based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-Beta Amyloid 40 Antibody, ß-amyloid 40, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI

Purification: Anti-Beta Amyloid 40 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Beta Amyloid 40 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus region of AB40.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-Beta Amyloid 40 antibody is useful for Western Blot and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.12 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Background: Background: Beta Amyloid protein is associated with neuronal injury and death in Alzheimer's disease. These proteins are the main components of amyloid plaques. Anti-Beta Amyloid 40 protein antibody is ideal for researchers interested in Apoptosis, Alzheimer's Research, Cell Cycle and Replication, Neurodegeneration, Neuroscience, and Neuronal Cell Marker research.

Gene ID: 351

NCBI Accession: NP_000475

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour