Stargazin antibody (AA 203-323)

Catalog No. ABIN1686631

Product Details anti-Stargazin Antibody

Target: Stargazin (CACNG2) (Calcium Channel, Voltage-Dependent, gamma Subunit 2 (CACNG2))

Binding Specificity: AA 203-323

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Stargazin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~35-55 kDa. Does not cross-react with TARPGamma3.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 203-323 (Cytoplasmic C-terminus) of rat TARPGamma2

Clone: S245-36

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1686631 was sufficient for detection of TARP Gamma2/4/8 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-Stargazin antibody (ABIN1686631)

Khodosevich, Jacobi, Farrow, Schulmann, Rusu, Zhang, Sprengel, Monyer, von Engelhardt: "Coexpressed auxiliary subunits exhibit distinct modulatory profiles on AMPA receptor function." in: Neuron, Vol. 83, Issue 3, pp. 601-15, (2014) (PubMed).

Target Details for Stargazin

Target: Stargazin (CACNG2) (Calcium Channel, Voltage-Dependent, gamma Subunit 2 (CACNG2))

Alternative Name: Stargazin (CACNG2 Products)

Synonyms: cacng2 antibody, zgc:64200 antibody, CACNG2 antibody, stargazin antibody, AW060990 antibody, B230105C07Rik antibody, B930041E13Rik antibody, stargazer antibody, stg antibody, wag antibody, waggler antibody, Ipr328 antibody, MRD10 antibody, calcium voltage-gated channel auxiliary subunit gamma 2 antibody, calcium channel, voltage-dependent, gamma subunit 2a antibody, calcium channel, voltage-dependent, gamma subunit 2 antibody, CACNG2 antibody, cacng2a antibody, cacng2 antibody, Cacng2 antibody

Background: Stargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state. There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.

Gene ID: 84347

NCBI Accession: NP_445803

UniProt: Q71RJ2

Pathways: Skeletal Muscle Fiber Development