Arp2/Arp3 (AA 301-394) antibody (Alexa Fluor 488)
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- Target
- Arp2/Arp3
- Binding Specificity
- AA 301-394
- Reactivity
- Mouse, Xenopus laevis
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Alexa Fluor 488
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Cross-Reactivity
- Mouse, Xenopus laevis
- Predicted Reactivity
- Human,Rat,Cow,Pig,Chicken
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human ARP2/ARP3
- Isotype
- IgG
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- Application Notes
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Arp2/Arp3
- Background
-
Synonyms: Actin like protein 2, Actin related protein 2, Actin-like protein 2, ACTIN-RELATED PROTEIN 2, ACTR2, ARP2 actin related protein 2 homolog yeast, ARP2, ARP2/3 COMPLEX, ARP2_HUMAN, OTTHUMP00000159937, OTTHUMP00000202270.
Background: Actin polymerization is required for a variety of cell functions, including chemotaxis, cell migration, cell adhesion, and platelet activation. Cells trigger actin polymerization through either the de novo nucleation of filaments from monomeric actin, the severing of existing filaments to create uncapped barbed ends, or the uncapping of existing barbed ends. The nucleation of actin is a rate-limiting and unfavorable reaction in actin polymerization and therefore requires the involvement of the Arp2/3 complex, which helps create new filaments and promotes the end-to-side cross-linking of actin filaments into the branching meshwork. The Arp2/3 complex consists of the actin-related proteins Arp2 and Arp3, and various other accessory proteins. The Arp2/3 complex promotes actin nucleation by binding the pointed end of actin filaments, or by associating with the side of an existing filament, and nucleates growth in the barbed direction. In addition, the Arp2/3 complex also mediates actin cytoskeletal outgrowths that are regulated by the Rho family of small GTPases. In response to GTP-binding Cdc42, the Arp2/3 complex binds the Cdc42 substrates, namely the WASP proteins, and initiates the formation of lamellipodia and filopodia.
- Gene ID
- 10097
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