POLH antibody (AA 51-160) (Cy3)

Catalog No. ABIN1702075

This Rabbit Polyclonal antibody specifically detects POLH in WB, IF (cc) and IF (p). It exhibits reactivity toward Human.

Quick Overview for POLH antibody (AA 51-160) (Cy3) (ABIN1702075)

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLH antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-POLH Antibody (Cy3)

Binding Specificity: AA 51-160

Predicted Reactivity: Human,Mouse,Rat,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA polymerase eta

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for POLH

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Alternative Name: DNA polymerase eta

Background:

Synonyms: DNA polymerase eta, FLJ16395, FLJ21978, POLH, POLH_HUMAN, polymerase DNA directed eta, RAD30, RAD30 homolog A, RAD30A, Xeroderma pigmentosum variant type protein, XP V, XPV.

Background: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.

Gene ID: 5429

Pathways: DNA Damage Repair