POLH antibody

Catalog No. ABIN7230724

This Rabbit Polyclonal antibody specifically detects POLH in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for POLH antibody (ABIN7230724)

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLH antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLH Antibody

Purpose: Rabbit Anti-POLH Polyclonal Antibody

Specificity: The antibody detects endogenous levels of POLH protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human POLH protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLH

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Alternative Name: POLH

Background: DNA polymerase eta, RAD30 homolog A, Xeroderma pigmentosum variant type proteinPOLH encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 78kD

Gene ID: 5429

UniProt: Q9Y253

Pathways: DNA Damage Repair