LRRFIP1 antibody (AA 121-220) (HRP)

Catalog No. ABIN1712359

Product Details anti-LRRFIP1 Antibody (HRP)

Target: LRRFIP1 (Leucine Rich Repeat (In FLII) Interacting Protein 1 (LRRFIP1))

Binding Specificity: AA 121-220

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRFIP1 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LRRFIP1

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LRRFIP1

Target: LRRFIP1 (Leucine Rich Repeat (In FLII) Interacting Protein 1 (LRRFIP1))

Alternative Name: LRRFIP1 (LRRFIP1 Products)

Synonyms: hm:zeh1367 antibody, lrrfip1 antibody, wu:fc06b04 antibody, wu:fi15c07 antibody, zeh1367 antibody, LRRFIP1 antibody, AU024550 antibody, Fliiap1 antibody, FLAP-1 antibody, FLAP1 antibody, FLIIAP1 antibody, GCF-2 antibody, GCF2 antibody, HUFI-1 antibody, TRIP antibody, leucine rich repeat (in FLII) interacting protein 1a antibody, LRR binding FLII interacting protein 1 antibody, leucine rich repeat (in FLII) interacting protein 1 antibody, lrrfip1a antibody, LRRFIP1 antibody, Lrrfip1 antibody

Background:

Synonyms: FLAP 1, FLAP1, FLIIAP 1, FLIIAP1, GC binding factor 2, GC-binding factor 2, GCF 2, GCF2, HUFI 1, HUFI1, Leucine rich repeat in FLII interacting protein 1, Leucine rich repeat flightless interacting protein 1, Leucine-rich repeat flightless-interacting protein 1, LRR FLII interacting protein 1, LRR FLII-interacting protein 1, LRRF1_HUMAN, LRRFIP 1, Lrrfip1, MGC10947, MGC119738, MGC119739, NEDD8 conjugating enzyme, TAR RNA interacting protein, TAR RNA-interacting protein, Transcription factor 9 like, TRIP.

Background: LRRFIP1 is an 738 amino acid human protein whose rodent counterpart is known as Lrrfip1 (also designated FLAP in mouse). LRRFIP1 is also believed to control smooth muscle cell proliferation following arterial injury through PDGF-A repression. The N-terminus of LRRFIP1 shows high homology to the coiled-coil domain of FLAP, a protein which binds the leucine-rich repeat (LRR) of Flightless I, and the interaction of LRRFIP1 with the LRR of Flightless I has been confirmed. LRRFIP1 does not bind single-stranded DNA or RNA significantly and binds double-stranded DNA weakly. In contrast, LRRFIP1 binds double-stranded RNA with high affinity, and two molecules of LRRFIP1 bind the TaR stem. The RNA binding domain has been identified and encompasses a lysine-rich motif. Flightless I has a C-terminal TaR-like domain which binds Actin and therefore the association of LRRFIP1 with the LRR of Flightless I may provide a link between the Actin cytoskeleton and RNA in mammalian cells.

Gene ID: 9208