PCK1 antibody (AA 201-300) (HRP)

Catalog No. ABIN1712374

This Rabbit Polyclonal antibody specifically detects PCK1 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for PCK1 antibody (AA 201-300) (HRP) (ABIN1712374)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PCK1 Antibody (HRP)

Binding Specificity: AA 201-300

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PCK1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background:

Synonyms: PCK1, PEPC, GTP, MGC22652, PEP carboxykinase, PEPCK 1, PEPCK1, PEPCKC, Phosphoenolpyruvate carboxykinase 1, Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase.

Background: PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized.

Gene ID: 5105

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis