DNAJB2 antibody (AA 21-120) (HRP)

Catalog No. ABIN1712555

Product Details anti-DNAJB2 Antibody (HRP)

Target: DNAJB2 (DnaJ (Hsp40) Homolog, Subfamily B, Member 2 (DNAJB2))

Binding Specificity: AA 21-120

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB2 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HSJ1/DNAJB2

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DNAJB2

Target: DNAJB2 (DnaJ (Hsp40) Homolog, Subfamily B, Member 2 (DNAJB2))

Alternative Name: HSJ1 (DNAJB2 Products)

Synonyms: MGC78895 antibody, DSMA5 antibody, HSJ-1 antibody, HSJ1 antibody, HSPF3 antibody, 2700059H22Rik antibody, Dnajb10 antibody, Hsj1 antibody, mDj8 antibody, DnaJ heat shock protein family (Hsp40) member B2 L homeolog antibody, DnaJ heat shock protein family (Hsp40) member B2 antibody, dnajb2.L antibody, DNAJB2 antibody, dnajb2 antibody, Dnajb2 antibody

Background:

Synonyms: Descriptions, DnaJ Hsp40 homolog subfamily B member 2, DnaJ homolog subfamily B member 2, DnaJ protein homolog 1, DNAJB2, Heat shock 40 kDa protein 3, Heat shock protein J1, Heat shock protein neuronal DNAJ like 1, HSJ 1, HSJ1, HSPF3, DNJB2_HUMAN.

Background: The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The presence of the J domain defines a protein as a member of the DnaJ family. DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. DnaJB2 (DnaJ homolog subfamily B member 2), also known as HSJ1 or HSPF3, is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus. Two isoforms are produced due to alternative splicing.

Gene ID: 3300